1. Oil is attached to a solid or sand mixture producing high flow resistance that clogs the operation 2. GreenZyme® is injected at 10% in a solution containing water 3. The GreenZyme® solution interacts with the sand increasing its wettability by attaching the water and enzyme solution to the sand and repelling the oil, creating mobility and decreasing its flow resistance. 4. Oil is released from the sand and becomes suitable for extraction. PTS LAB Where: PTS Laboratories, Houston, Texas. File # 22507, done on October 2005.. How: Oil source: Brazilian API 20 oil with medium wax content. GreenZyme® used: 3%, 5% and 7% strength. Core sample: 1.50 inch diameter Berea core sample for GreenZyme® core flood under overburden conditions., i.e. oil sample was heated to 180 degree F for two weeks and under 1000 psi net confining stress. GreenZyme® flood: 5 pore volumes of diluted strength flooded the core, flow stop and a 48 hour soak follow, then 48 hour flow of simulated brine until 99.9% water-cut reached. Initial water saturation, oil saturation, permeability to oil at initial water saturation, residual oil saturation, oil produced (OOIP), and permeability to water at residual oil saturation were determined. Oil volume was continuously monitored and recorded. Repeat for different % strength of GreenZyme®. Results: Over 12.7% of additional oil produced (OOIP) by GreenZyme®. BTP - Greenzyme tube 2 BTP - Greenzyme tube 1